mouse igg2b ab Search Results


negative control for mouse igg2b ab-1  (Thermo Fisher)
N/A
Take advantage of a quality product that delivers accurate results with the Thermo Scientific™ Negative Control for Mouse IgG2b Ab-1, Mouse Monoclonal Antibody.
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mouse igg2b κ isotype control pe  (Elabscience Biotechnology)
91
Elabscience Biotechnology mouse igg2b κ isotype control pe
Mouse Igg2b κ Isotype Control Pe, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+igg2b+ab/pm37217247-90-37-69?v=Elabscience+Biotechnology
Average 91 stars, based on 1 article reviews
mouse igg2b κ isotype control pe - by Bioz Stars, 2026-08
91/100 stars
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zmab-mouse igg2b (cmigg  (AB Biosciences)
90
AB Biosciences zmab-mouse igg2b (cmigg
Significant increase of CR Abs <t>IgG</t> in moderate/severe COVID-19 patients’ sera may contribute to cytokine secretion and NET formation in human leukocytes in the presence of RBD. IgG against nucleocapsid, N, RBD and ACE2 proteins in sera from COVID-19 patients and healthy donors (HD) were measured by ELISA. Comparison of ( A ) the levels of IgG against N, ( B ) RBD, and ( C ) ACE2 in mild vs. moderate/severe COVID-19 patients. ( D ) Anti-ACE2 IgG levels in sera of mild vs. moderate/severe COVID-19 patients after BSA or RBD preadsorption. For the leukocyte activation test, isolated human leukocytes were preincubated with 1:100 diluted HD serum or sera from CR Ab-positive COVID-19 patients (CR serum) as indicated in the presence or absence of additional rACE2 (10 μg/mL) for 30 min. Afterward, unbound antibodies were removed by centrifugation, and the cells were treated with the RBD protein (10 μg/mL) for 24 h. The supernatants were collected to measure the ( E ) TNF-α, ( F ) IL-1β, ( G ) IL-6, ( H ) IL-8, and ( I ) MPO levels using ELISA kits. The averages of triplicate cultures ± SD are shown. Multiple comparison of antibody response and leukocyte activation tests ( n = 3–5) was conducted by one-way ANOVA and Tukey’s post hoc test. The comparison between BSA and RBD preadsorption was analyzed by paired Student’s t-test; P values were displayed, and for values less than 0.0001, they were represented as ****
Zmab Mouse Igg2b (Cmigg, supplied by AB Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+igg2b+ab/pmc11027296-51-0-10?v=AB+Biosciences
Average 90 stars, based on 1 article reviews
zmab-mouse igg2b (cmigg - by Bioz Stars, 2026-08
90/100 stars
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goat anti mouse igg2b pe 2ndary ab  (Tonbo Biosciences)
N/A
Goat anti Mouse IgG2b Secondary antibody Conjugated PE
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Significant increase of CR Abs IgG in moderate/severe COVID-19 patients’ sera may contribute to cytokine secretion and NET formation in human leukocytes in the presence of RBD. IgG against nucleocapsid, N, RBD and ACE2 proteins in sera from COVID-19 patients and healthy donors (HD) were measured by ELISA. Comparison of ( A ) the levels of IgG against N, ( B ) RBD, and ( C ) ACE2 in mild vs. moderate/severe COVID-19 patients. ( D ) Anti-ACE2 IgG levels in sera of mild vs. moderate/severe COVID-19 patients after BSA or RBD preadsorption. For the leukocyte activation test, isolated human leukocytes were preincubated with 1:100 diluted HD serum or sera from CR Ab-positive COVID-19 patients (CR serum) as indicated in the presence or absence of additional rACE2 (10 μg/mL) for 30 min. Afterward, unbound antibodies were removed by centrifugation, and the cells were treated with the RBD protein (10 μg/mL) for 24 h. The supernatants were collected to measure the ( E ) TNF-α, ( F ) IL-1β, ( G ) IL-6, ( H ) IL-8, and ( I ) MPO levels using ELISA kits. The averages of triplicate cultures ± SD are shown. Multiple comparison of antibody response and leukocyte activation tests ( n = 3–5) was conducted by one-way ANOVA and Tukey’s post hoc test. The comparison between BSA and RBD preadsorption was analyzed by paired Student’s t-test; P values were displayed, and for values less than 0.0001, they were represented as ****

Journal: Journal of Biomedical Science

Article Title: Enhancement of NETosis by ACE2-cross-reactive anti-SARS-CoV-2 RBD antibodies in patients with COVID-19

doi: 10.1186/s12929-024-01026-5

Figure Lengend Snippet: Significant increase of CR Abs IgG in moderate/severe COVID-19 patients’ sera may contribute to cytokine secretion and NET formation in human leukocytes in the presence of RBD. IgG against nucleocapsid, N, RBD and ACE2 proteins in sera from COVID-19 patients and healthy donors (HD) were measured by ELISA. Comparison of ( A ) the levels of IgG against N, ( B ) RBD, and ( C ) ACE2 in mild vs. moderate/severe COVID-19 patients. ( D ) Anti-ACE2 IgG levels in sera of mild vs. moderate/severe COVID-19 patients after BSA or RBD preadsorption. For the leukocyte activation test, isolated human leukocytes were preincubated with 1:100 diluted HD serum or sera from CR Ab-positive COVID-19 patients (CR serum) as indicated in the presence or absence of additional rACE2 (10 μg/mL) for 30 min. Afterward, unbound antibodies were removed by centrifugation, and the cells were treated with the RBD protein (10 μg/mL) for 24 h. The supernatants were collected to measure the ( E ) TNF-α, ( F ) IL-1β, ( G ) IL-6, ( H ) IL-8, and ( I ) MPO levels using ELISA kits. The averages of triplicate cultures ± SD are shown. Multiple comparison of antibody response and leukocyte activation tests ( n = 3–5) was conducted by one-way ANOVA and Tukey’s post hoc test. The comparison between BSA and RBD preadsorption was analyzed by paired Student’s t-test; P values were displayed, and for values less than 0.0001, they were represented as ****

Article Snippet: ZMAB-mouse IgG2b (cmIgG) was used as an isotype-matched antibody control (AB Biosciences, Concord, MA).

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Activation Assay, Isolation, Centrifugation

CR IgG purified from unvaccinated COVID-19 patients induces neutrophil activation and NETosis in the presence of RBD. Isolated human neutrophils were preincubated with 100 μg/mL purified IgG from HD serum (HD IgG) or different COVID-19 patient sera: anti-RBD IgG (anti-ACE2 antibody negative but anti-RBD antibody positive), anti-ACE2 IgG (anti-ACE2 antibody positive), or CR IgG (CR antibody positive) in the presence or absence of additional rACE2 (10 μg/mL) or dasatinib (50 nM) as indicated for 30 min. After removing unbound antibodies by centrifugation, cells were treated with the RBD protein (10 μg/mL) for 24 h. ( A ) IL-8 and ( B ) MPO levels in the supernatants were measured using ELISA kits. ( C , D ) Cell suspensions were cytocentrifuged onto microscope slides, fixed, and subjected to immunofluorescence staining with anti-MPO antibody (green), anti-histone antibody (red), and Hoechst nuclear stain (blue). The red arrow indicates neutrophils that released NETs. The area of NET formation was quantified using ImageJ. Averages of triplicate cultures ± SD are shown. Views for NET quantification were randomly selected with 9 pictures from each experiment. Statistical significance was assessed using one-way ANOVA and Tukey’s post hoc test; **** p < 0.0001. Bar: 25 μm

Journal: Journal of Biomedical Science

Article Title: Enhancement of NETosis by ACE2-cross-reactive anti-SARS-CoV-2 RBD antibodies in patients with COVID-19

doi: 10.1186/s12929-024-01026-5

Figure Lengend Snippet: CR IgG purified from unvaccinated COVID-19 patients induces neutrophil activation and NETosis in the presence of RBD. Isolated human neutrophils were preincubated with 100 μg/mL purified IgG from HD serum (HD IgG) or different COVID-19 patient sera: anti-RBD IgG (anti-ACE2 antibody negative but anti-RBD antibody positive), anti-ACE2 IgG (anti-ACE2 antibody positive), or CR IgG (CR antibody positive) in the presence or absence of additional rACE2 (10 μg/mL) or dasatinib (50 nM) as indicated for 30 min. After removing unbound antibodies by centrifugation, cells were treated with the RBD protein (10 μg/mL) for 24 h. ( A ) IL-8 and ( B ) MPO levels in the supernatants were measured using ELISA kits. ( C , D ) Cell suspensions were cytocentrifuged onto microscope slides, fixed, and subjected to immunofluorescence staining with anti-MPO antibody (green), anti-histone antibody (red), and Hoechst nuclear stain (blue). The red arrow indicates neutrophils that released NETs. The area of NET formation was quantified using ImageJ. Averages of triplicate cultures ± SD are shown. Views for NET quantification were randomly selected with 9 pictures from each experiment. Statistical significance was assessed using one-way ANOVA and Tukey’s post hoc test; **** p < 0.0001. Bar: 25 μm

Article Snippet: ZMAB-mouse IgG2b (cmIgG) was used as an isotype-matched antibody control (AB Biosciences, Concord, MA).

Techniques: Purification, Activation Assay, Isolation, Centrifugation, Enzyme-linked Immunosorbent Assay, Microscopy, Immunofluorescence, Staining

Vaccination is conducive to decreasing CR Abs IgG levels in the sera of COVID-19 patients. IgG against ( A ) RBD, ( B ) nucleocapsid, N and ( C ) ACE2 proteins in sera from different groups were measured by ELISA. ( D ) Anti-ACE2 IgG levels in sera of unvaccinated COVID-19 patients and vaccinated COVID-19 patients after BSA or RBD preadsorption. Multiple comparisons in COVID-19 samples were conducted by two-way ANOVA. The comparison between BSA and RBD preadsorption was analyzed by paired Student’s t-test; P values were displayed, and for values less than 0.0001, they were represented as ****

Journal: Journal of Biomedical Science

Article Title: Enhancement of NETosis by ACE2-cross-reactive anti-SARS-CoV-2 RBD antibodies in patients with COVID-19

doi: 10.1186/s12929-024-01026-5

Figure Lengend Snippet: Vaccination is conducive to decreasing CR Abs IgG levels in the sera of COVID-19 patients. IgG against ( A ) RBD, ( B ) nucleocapsid, N and ( C ) ACE2 proteins in sera from different groups were measured by ELISA. ( D ) Anti-ACE2 IgG levels in sera of unvaccinated COVID-19 patients and vaccinated COVID-19 patients after BSA or RBD preadsorption. Multiple comparisons in COVID-19 samples were conducted by two-way ANOVA. The comparison between BSA and RBD preadsorption was analyzed by paired Student’s t-test; P values were displayed, and for values less than 0.0001, they were represented as ****

Article Snippet: ZMAB-mouse IgG2b (cmIgG) was used as an isotype-matched antibody control (AB Biosciences, Concord, MA).

Techniques: Enzyme-linked Immunosorbent Assay, Comparison